反応 #738525

反応方程式

(8-naphthalen-1-ylmethyl-4-oxo-1-phenyl-1,3,8-triaza-spiro[4.5]dec-3-yl)-acetic acid

diisopropylcarbodiimide

1-hydroxy-1H-benzotriazol

Fmoc-glycine

acetic acid

sodium methoxide

diisopropylethylamine

→

title compound

(8-Naphthalen-1-ylmethyl-4-oxo-1-phenyl-1.3,8-triaza-spiro[4.5]dec-3-yl)-acetylglycine methyl ester

溶媒

dimethyl formamide

dimethyl formamide

tetrahydrofuran methanol

tetrahydrofuran methanol

反応条件

後処理

1. 1
   その他was placed in a Teflon tube
2. 2
   その他equipped with a frit on a mechanical shaker
3. 3
   その他The solvent was removed by suction
4. 4
   その他The solution was removed by suction
5. 5
   洗浄the resin was washed with N,N-dimethylformamide (3×1.5 ml)
6. 6
   workup.STIRRINGthe mixture was stirred at room temperature for 0.5 h
7. 7
   workup.STIRRINGthis was shaken at room temperature ovemight
8. 8
   ろ過The resin was filtered
9. 9
   洗浄washed with dimethylformamide (2×1.5 ml), dichloromethane (4×1.5 ml), methanol (2×1.5 ml) and tetrahydrofuran/methanol 4:1 (2×1.5 ml)
10. 10
    workup.ADDITIONwas added to the resin
11. 11
    workup.STIRRINGthe suspension was agitated at 50 ° C. for 16 h
12. 12
    洗浄the resin was washed with tetrahydrofuran (1 ml)
13. 13
    濃縮The combined filtrates were concentrated in vacuo

実験手順

Wang resin loaded with Fmoc-glycine (88 mg, 0.045 mmol) was placed in a Teflon tube equipped with a frit on a mechanical shaker. The resin was allowed to swell in dimethyl formamide (1.5 ml) for 1 h. The solvent was removed by suction and the resin was agitated with 20% piperidine in N,N-dimethylformamide (1.5 ml) for 30 minutes. The solution was removed by suction and the resin was washed with N,N-dimethylformamide (3×1.5 ml). To a solution of (8-naphthalen-1-ylmethyl-4-oxo-1-phenyl-1,3,8-triaza-spiro[4.5]dec-3-yl)-acetic acid (0.0773 g, 0.18 mmol) in dimethyl formamide (1.6 ml), diisopropylcarbodiimide (29 μl, 0.18 mmol) and 1-hydroxy-1H-benzotriazol (0.0243 g, 0.18 mmol) were added and the mixture was stirred at room temperature for 0.5 h. The resulting solution and diisopropylethylamine (31 μl, 0.18 mmol) were added to the above resin and this was shaken at room temperature ovemight. The resin was filtered and washed with dimethylformamide (2×1.5 ml), dichloromethane (4×1.5 ml), methanol (2×1.5 ml) and tetrahydrofuran/methanol 4:1 (2×1.5 ml). A solution of sodium methoxide (0.009 mmol) in a mixture of tetrahydrofuran/methanol 4:1 (2 ml) was added to the resin and the suspension was agitated at 50 ° C. for 16 h. The mixture was neutralized by addition of a solution of acetic acid (0.01 mmol) in a mixture of tetrahydrofuran/methanol 4:1 (1 ml), the solution was drained and the resin was washed with tetrahydrofuran (1 ml). The combined filtrates were concentrated in vacuo to afford the title compound.

出典