反応 #65357

ord-24624ba4ad42404e88c2b51eb4f0dcd5

反応方程式

Nc1ncnc2c1ncn2[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1F
6-Amino-9-(2-deoxy-2-fluoro-β-D-ribofuranosyl)-9H-purine
Nc1ncnc2c1ncn2[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O
adenosine
→
O=c1[nH]cnc2c1ncn2[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1F
title compound
O=c1[nH]cnc2c1ncn2[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1F
9-(2-Deoxy-2-fluoro-β-D-ribofuranosyl)hypoxanthine

反応物

Nc1ncnc2c1ncn2[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1F
6-Amino-9-(2-deoxy-2-fluoro-β-D-ribofuranosyl)-9H-purine
Nc1ncnc2c1ncn2[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O
adenosine

試薬

なし

溶媒

O
water

反応条件

詳細条件
See reaction.notes.procedure_details.

後処理

  1. 1
    その他The solvent was removed under vacuum
  2. 2
    workup.DISSOLUTIONThe residue was dissolved in water/n-propanol (7/3)
  3. 3
    その他chromatographed on a 5×90 cm column of Sephadex
  4. 4
    その他the solvent removed under vacuum
  5. 5
    workup.DISSOLUTIONThe residue was dissolved in water

実験手順

6-Amino-9-(2-deoxy-2-fluoro-β-D-ribofuranosyl)-9H-purine (0.29 g, 1.0 mmole), prepared as in Example 8, was dissolved in 100 ml of water. Calf intestinal adenosine deaminase (4 I.U., Boehringer Mannheim) was added and the solution incubated at 37° C. for 24 hrs. The solvent was removed under vacuum. The residue was dissolved in water/n-propanol (7/3) and chromatographed on a 5×90 cm column of Sephadex G-10 (Pharmacia LKB) with water/n-propanol (7/3) as the solvent. Product-containing fractions were combined and the solvent removed under vacuum. The residue was dissolved in water and lyophilized to obtain title compound that analyzed as a hydrate.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: US05420115uspto-grants-1995_05