反応 #65346

反応方程式

2,6-Diamino-9-(2-deoxy-2-fluoro-β-D-ribofuranosyl)-9H-purine

adenosine

→

title compound

9-(2-Deoxy-2-fluoro-β-D-ribofuranosyl)guanine

溶媒

water

反応条件

後処理

1. 1
   workup.WAITAfter 3 hrs
2. 2
   ろ過the suspension was filtered
3. 3
   その他to remove the first batch of product crystals
4. 4
   温度the suspension cooled to 4° C
5. 5
   ろ過The suspension was filtered
6. 6
   その他to remove the second batch of product crystals

実験手順

2,6-Diamino-9-(2-deoxy-2-fluoro-β-D-ribofuranosyl)-9H-purine (0.20 g, 0.64 mmole), prepared as in Example 6, was dissolved in 15 ml of water. Calf intestinal adenosine deaminase (4 I.U., Boehringer Mannheim) was added and the solution incubated at 37° for 4 days. The solution was cooled to 4° C. After 3 hrs, the suspension was filtered to remove the first batch of product crystals. The volume of the filtrate was reduced under vacuum and the suspension cooled to 4° C. The suspension was filtered to remove the second batch of product crystals. The batches of product crystals were combined, suspended in water and lyophilized to obtain title compound that analyzed as a 1.3 hydrate.

出典