反応 #65116

反応方程式

NaOH

N-benzoyl-L-aspartic acid

L-phenylalanine methyl ester

acetonitrile

→

N-benzoyl-L-aspartyl-L-phenylalanine methyl ester

溶媒

water

反応条件

後処理

1. 1
   ろ過filtered immobilized enzyme
2. 2
   その他prepared above
3. 3
   その他After completion of the reaction
4. 4
   その他the immobilized enzyme was separated by filtration
5. 5
   その他Resulting slurry
6. 6
   ろ過was filtered
7. 7
   洗浄washed with water

実験手順

1.19 g (5.0 mmol) of N-benzoyl-L-aspartic acid and 1.79 g (10.0 mmol) of L-phenylalanine methyl ester were added to a flask which contained 50 ml of 50% acetonitrile in water containing 5 mM CaCl2. The pH of the solution was adjusted to 6.0 with 10M NaOH. The solution was admixed with 4 g of suction filtered immobilized enzyme prepared above. Thereafter, the reaction were carried out with stirring for 4 hours at 40° C. After completion of the reaction, the immobilized enzyme was separated by filtration. The pH of the filtrate was then adjusted to 3.7 by 10 molar hydrochloric acid. Resulting slurry was filtered and washed with water to give N-benzoyl-L-aspartyl-L-phenylalanine methyl ester.

出典