反応 #634341

ord-ec5a45679f67495eab35811709f4f2aa

反応方程式

O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate
C=C(OP(=O)(O)O)C(=O)O
phosphoenolpyruvate
N[C@@H](CCC(=O)[O-])C(=O)[O-]
glutamate
→
N[C@@H](CCC(=O)O)C(=O)O
L-Glutamic Acid

反応物

O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate
C=C(OP(=O)(O)O)C(=O)O
phosphoenolpyruvate
N[C@@H](CCC(=O)[O-])C(=O)[O-]
glutamate

試薬

なし

反応条件

詳細条件
See reaction.notes.procedure_details.

後処理

  1. 1
    workup.ADDITIONwere introduced into the SC17sucA strain

実験手順

Subsequently, a citrate synthase gene, a phosphoenolpyruvate carboxylase gene and a glutamate dehydrogenase gene derived from Escherichia coli were introduced into the SC17sucA strain.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: USRE042350E1uspto-grants-2011_05