反応 #624525

反応方程式

octahydrocyclopenta[c]pyrrole hydrochloride

NaCN

NaOH

NaOH

→

octahydrocyclopenta[c]pyrrole-1-carbonitrile

収率 85.6%

溶媒

potassium phosphate

potassium phosphate

反応条件

後処理

1. 1
   workup.ADDITIONwas added 30 μL of A
2. 2
   温度was maintained at 7.5 via feedback
3. 3
   workup.STIRRINGAfter stirring for 48 hours
4. 4
   温度raising the pH to ˜9.9
5. 5
   workup.STIRRINGAfter stirring at room temperature (about 21° C.) for an additional 1 hour
6. 6
   抽出the mixture was extracted with ethyl acetate
7. 7
   その他After phase separation and solvent evaporation

実験手順

To a 50-mL 3-neck flask under air was added 25 mL of 100 mM pH 8.0 potassium phosphate buffer and 400 mg of octahydrocyclopenta[c]pyrrole hydrochloride, 500 mg of Na2S2O5 and the pH was adjusted to approximately 7.5 with 10N NaOH. To the pH adjusted solution was added 30 μL of A. niger catalase suspension (Sigma Aldrich; catalog number C-3515) and 300 mg of monoamine oxidase powder (prepared by the method of Example 2 (with SEQ ID NO 10 in pH 8.0 potassium phosphate buffer. The resulting pale yellow solution was stirred under air for 24 hours during which time the pH was maintained at 7.5 via feedback controlled addition of 1N NaOH in 1-5 μL portions. After stirring for 48 hours, 300 mg of NaCN was added to the reaction mixture raising the pH to ˜9.9. After stirring at room temperature (about 21° C.) for an additional 1 hour, the mixture was extracted with ethyl acetate. After phase separation and solvent evaporation, 316 mg of octahydrocyclopenta[c]pyrrole-1-carbonitrile was isolated (82% yield). 1H-NMR showed ˜90% (1S,3aR,6aS), “trans” and ˜10% of the (1R,3aR,6aS) epimer, “cis.” (1H-NMR (300 MHz, CDCl3) spectrum: δ 3.95 (d, J=6.6, cis aminonitrile methine H), 3.62 (d, J=1.2; trans aminonitrile methine H), 3.15 (m, 1H), 2.71 (m, 2H), 2.62 (m, 1H), 1.63-1.92 (m, 3H), 1.55 (m, 1H), 1.22-1.45 (m, 3H)).

出典