反応 #5857

反応方程式

4-aminoantipyrine

uricase

→

Uric Acid

溶媒

phosphate

反応条件

後処理

1. 1
   workup.ADDITIONTo each of the mixtures was added a reagent
2. 2
   その他prepared
3. 3
   その他using purified water as a control
4. 4
   その他The results obtained

実験手順

To each of the mixtures was added a reagent prepared by dissolving 1 μmol 4-aminoantipyrine and 2 units of uricase in 1.5 ml of 0.1M phosphate buffer (pH 6.5). After the resulting mixtures were maintained in a thermostat at 37° C. for about 5 minutes, the absorbance was measured at a wavelength of 555 nm with a spectrophotometer (Model 228, manufactured by Hitachi, Ltd.) using purified water as a control. The results obtained are shown in Table 3.

出典