反応 #563771

ord-ec4b76b4beb443f0b3ee53df3e72b8aa

反応方程式

O=C(N[C@H](CO)[C@H](O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl
chloramphenicol
O=C(N[C@H](CO)[C@H](O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl
chloramphenicol
O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
biotin
O=S(=O)([O-])[O-].[NH4+].[NH4+]
ammonium sulfate
NC(N)=O
urea
O.O.O.O.O.O.O.O=S(=O)([O-])[O-].[Mg+2]
magnesium sulfate heptahydrate
Cc1ncc(C[n+]2csc(CCO)c2C)c(N)n1.Cl.[Cl-]
thiamine hydrochloride
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
→
N[C@@H](Cc1c[nH]c2ccccc12)C(=O)O
tryptophan

反応物

O=C(N[C@H](CO)[C@H](O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl
chloramphenicol
O=C(N[C@H](CO)[C@H](O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl
chloramphenicol
O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
biotin
O=S(=O)([O-])[O-].[NH4+].[NH4+]
ammonium sulfate
NC(N)=O
urea
O.O.O.O.O.O.O.O=S(=O)([O-])[O-].[Mg+2]
magnesium sulfate heptahydrate
—
dihydrogen potassium phosphate
Cc1ncc(C[n+]2csc(CCO)c2C)c(N)n1.Cl.[Cl-]
thiamine hydrochloride
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
—
MnSO4.4H2O
—
FeSO4.7H2O

試薬

なし

溶媒

C[C@@H](O)[C@H](N)C(=O)O
threonine
C[C@@H](O)[C@H](N)C(=O)O
Thr

反応条件

詳細条件
See reaction.notes.procedure_details.

実験手順

After culturing at 30° C. for 2 weeks, about 10,000 colonies resistant to chloramphenicol appeared, which were replicated in threonine-free medium (Thr deficient medium) which contained 2% of glucose, 1% of ammonium sulfate, 0.25% of urea, 0.1% of dihydrogen potassium phosphate, 0.04% of magnesium sulfate heptahydrate, 1 mg/dl of FeSO4.7H2O, 1 mg/dl of MnSO4.4H2O, 200 μg/dl of thiamine hydrochloride and 50 μg/dl of biotin, pH 7.0, 1.8% of agar, and 3 strains which were resistant to chloramphenicol and tryptophan independent were obtained.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: US04885245uspto-grants-1989_12