反応 #552352

反応方程式

2-[4-(p-toluenesulphonylthio)-3-phenoxyacetamido-2-oxoazetidin-1-yl]-3-methylenebutyric acid p-nitrobenzyl ester

sodium bisulphite

→

2 -[4-(p-toluenesulphonylthio)-3-phenoxyacetamido-2-oxoazetidin-1-yl]-3-hydroxycrotonic acid p-nitrobenzyl ester

溶媒

ethyl acetate

methyl acetate

water

反応条件

後処理

1. 1
   その他ozonised at -70° C.
2. 2
   温度the latter is warmed to 0°-5° C
3. 3
   その他the aqueous phase is separated off
4. 4
   洗浄the organic phase is washed with water
5. 5
   乾燥dried over magnesium sulphate
6. 6
   workup.DISSOLUTIONThe crude product is dissolved in 3 ml of methylene chloride
7. 7
   workup.ADDITION15 ml of toluene are added
8. 8
   ろ過The precipitate is filtered off
9. 9
   その他the filtrate is evaporated in vacuo
10. 10
    その他The residue is recrystallised from methanol

実験手順

(di) The crude 2-[4-(p-toluenesulphonylthio)-3-phenoxyacetamido-2-oxoazetidin-1-yl]-3-methylenebutyric acid p-nitrobenzyl ester, obtained according to Example 1.(cvii), is dissolved in 20 ml of methyl acetate and ozonised at -70° C. until no further starting material is present, according to a thin layer chromatogram. A stream of nitrogen is then passed through the solution and the latter is warmed to 0°-5° C. A solution of 300 mg of sodium bisulphite in 5 ml of water is added and the mixture is stirred for about 5 minutes until no further ozonide can be detected with potassium iodide/starch paper. The mixture is diluted with ethyl acetate, the aqueous phase is separated off, and the organic phase is washed with water, dried over magnesium sulphate and freed from solvent in vacuo. The crude product is dissolved in 3 ml of methylene chloride and 15 ml of toluene are added. The precipitate is filtered off and the filtrate is evaporated in vacuo. The residue is recrystallised from methanol and gives 2 -[4-(p-toluenesulphonylthio)-3-phenoxyacetamido-2-oxoazetidin-1-yl]-3-hydroxycrotonic acid p-nitrobenzyl ester of melting point 159°-160° C.

出典