反応 #524871

反応方程式

dicyclohexylcarbodiimide

Nα-t-butoxycarbonyl-O-benzyl-L-tyrosyl-D-alanine

Nα-t-butoxycarbonyl-O-benzyl-D-serine

Nα-t-butoxycarbonyl-L-tryptophan

dicyclohexylcarbodiimide

→

Nαt-Butoxycarbonyl-L-tryptophyl-O-benzyl-D-seryl-O-benzyl-L-tyrosyl-D-alanine

反応条件

後処理

1. 1
   洗浄The resin is washed with ethanol
2. 2
   その他dried
3. 3
   ろ過The mixture is filtered
4. 4
   その他the methanol solution evaporated

実験手順

Nαt-Butoxycarbonyl-L-tryptophyl-O-benzyl-D-seryl-O-benzyl-L-tyrosyl-D-alanine resin is prepared from 8 g. of Nα-t-butoxycarbonyl-O-benzyl-L-tyrosyl-D-alanine resin by successive coupling, according to the general process for solid phase synthesis given in Example 1, with (13 g., 9.8 mmol, of Nα-t-butoxycarbonyl-O-benzyl-D-serine and 2 g., 9.7 mmol, of dicyclohexylcarbodiimide and (2) 3 g., 9.8 mmol, of Nα-t-butoxycarbonyl-L-tryptophan and 2 g. of dicyclohexylcarbodiimide. The resin is washed with ethanol and dried. It is then stirred for twenty-four hours at 25° C. with 200 ml. of methanol and 20 ml. of triethylamine. The mixture is filtered and the methanol solution evaporated. The residue is solidified from ether-petroleum ether; 2 g.; m.p. 155°-156° C.

出典