反応 #5242

反応方程式

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride

[(amino)carbonyl]amino-1-(β-D-ribofuranosyl)-1H-imidazole-4-carboxylic acid

→

oxanosine

溶媒

water

CH3OH

反応条件

後処理

1. 1
   その他The reaction was quenched by addition of excess 60% aqueous acetic acid
2. 2
   workup.WAITAfter 30 minutes
3. 3
   workup.ADDITIONthe mixture was diluted with water
4. 4
   濃縮concentrated
5. 5
   その他to remove CH3OH
6. 6
   その他The resulting powder (100 mg) was chromatographed on reverse phase silica gel [
7. 7
   洗浄10 g, flash, stepwise gradient elution
8. 8
   workup.ADDITIONFractions containing the product
9. 9
   濃縮concentrated under reduced pressure

実験手順

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (78 mg, 0.41 mmol) was added to a solution of 5-[[(amino)carbonyl]amino-1-(β-D-ribofuranosyl)-1H-imidazole-4-carboxylic acid (19 mg, 0.063 mmol) in water (2 mL) and CH3OH (5 mL) and the mixture was stirred at room temperature overnight. The reaction was quenched by addition of excess 60% aqueous acetic acid. After 30 minutes, the mixture was diluted with water, concentrated to remove CH3OH and lyophilized. The resulting powder (100 mg) was chromatographed on reverse phase silica gel [10 g, flash, stepwise gradient elution: water (200 mL), 10% CH3OH in water (v/v, 100 mL), 20% CH3OH in water (v/v, 100 mL), 10-mL fractions]. Fractions containing the product were combined, concentrated under reduced pressure and lyophilized to give oxanosine: 16 mg (89%).

出典