反応 #5238
ord-29553a4becdf4d34adb98b5354444849
反応条件
詳細条件
See reaction.notes.procedure_details.
後処理
- 1その他0.5 mL aliquots of the membrane preparation
- 2ろ過samples were filtered rapidly through Whatman GF/C glass filters under negative pressure
- 3洗浄washed three times with ice-cold binding buffer (5 mL)
実験手順
0.5 mL aliquots of the membrane preparation were incubated with unlabeled drugs, and 0.15 nM [3H]spiperone in a final volume of 1 mL containing 50 mM Tris HCl, 120 mM NaCl and 1 mM MgCl2 (pH 7.7). Nonspecific binding was measured in the presence of 100 nM (+)-butaclamol. After 15 minutes of incubation at 37° C., samples were filtered rapidly through Whatman GF/C glass filters under negative pressure, and washed three times with ice-cold binding buffer (5 mL).