反応 #520236

ord-0a4c5d40d25443239827f11fa4bf233a

反応方程式

CN(C)[C@@H]1C(O)=C(C(N)=O)C(=O)[C@@]2(O)C(O)=C3C(=O)c4c(O)cccc4[C@@](C)(O)[C@H]3C[C@@H]12
tetracycline
CC(C)(CO)[C@@H](O)C(=O)NCCC(=O)[O-]
pantothenate
NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O
kanamycin
CC[C@H](C)[C@H](N)C(=O)O
isoleucine
→
CC(C)(CO)[C@@H](O)C(=O)NCCC(=O)O
Pantothenic Acid

反応物

CN(C)[C@@H]1C(O)=C(C(N)=O)C(=O)[C@@]2(O)C(O)=C3C(=O)c4c(O)cccc4[C@@](C)(O)[C@H]3C[C@@H]12
tetracycline
CC(C)(CO)[C@@H](O)C(=O)NCCC(=O)[O-]
pantothenate
NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O
kanamycin
CC[C@H](C)[C@H](N)C(=O)O
isoleucine

試薬

なし

反応条件

詳細条件
See reaction.notes.procedure_details.

実験手順

The formation of pantothenate by the C. glutamicum strains ATCC13032ΔilvA/pND-D2 and ATCC13032ΔilvA/pND-D2, pT-pfkAexp was tested in medium CGXII (Keilhauer et al., 1993, Journal of Bacteriology, 175:5595-5603; table 1), which had been supplemented with 25 μg/ml kanamycin, 2 mM isoleucine and in the case of the strain ATCC13032ΔilvA/pND-D2, pT-pfkAexp with additionally 10 μg/ml tetracycline.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: US06667166B2uspto-grants-2003_12