反応 #502066

反応方程式

phenyllactic acid

phenylglycine

2-chloromandelic acid

→

(S)-phenyllactic Acid

反応条件

後処理

1. 1
   濃縮However, in this case, adjustment of the solvent concentration in the nonchiral HPLC method
2. 2
   その他Representative nonchiral chromatograms of the product (1.9 min) and cyanohydrin substrate (3.7 min)
3. 3
   その他at 6 min.

実験手順

The analysis of phenyllactic acid was initially plagued with the same problems discussed for phenylglycine and 2-chloromandelic acid. However, in this case, adjustment of the solvent concentration in the nonchiral HPLC method led to a shift in the retention time of the acid, so that it no longer coeluted with the cell lysate peak. Following this, no problems were encountered with either the nonchiral or chiral methods. Representative nonchiral chromatograms of the product (1.9 min) and cyanohydrin substrate (3.7 min) are shown in FIG. 8A, while the chiral analysis of the acid is shown in FIG. 8B, with the L-enantiomer eluting at 2 min and the opposite enantiomer at 6 min.

出典