反応 #4986

反応方程式

Alkynylamino nucleoside

5-(3-trifluoroacetamido-1-propynyl)-2', 3'-dideoxyuridine

triphosphate

→

5-(3-amino-1-propynyl)-2', 3'-dideoxyuridine 5'-triphosphate

反応条件

後処理

1. 1
   その他was removed
2. 2
   workup.ADDITIONAfter addition of the second aliquot of phosphorus oxychloride, phosphorylation
3. 3
   その他Assuming an absorption coefficient for the product equal to that of the starting material (13,000), the yield of triphosphate 23, based on its UV absorption at 291.5 nm

実験手順

Alkynylamino nucleoside 22 (0.30 mmol) was converted to the corresponding triphosphate and its trifluoroacetyl group was removed following the general procedure given in Example 5E. After addition of the second aliquot of phosphorus oxychloride, phosphorylation was allowed to proceed for 210 minutes. Assuming an absorption coefficient for the product equal to that of the starting material (13,000), the yield of triphosphate 23, based on its UV absorption at 291.5 nm, was 18%.

出典