反応 #473540

反応方程式

formaldehyde

potassium phosphate

α-benzyl-DL-serine

pyridoxal phosphate

→

2-Benzylserine

反応条件

後処理

1. 1
   その他A reaction mixture in a total amount of 0.1 mL obtained
2. 2
   その他was reacted at 30° C. for 10 minutes

実験手順

A reaction mixture in a total amount of 0.1 mL obtained by adding 0.03 mL of the cell-free extract solution to a reaction solution A having a composition of 50 mM potassium phosphate buffer (pH 7.4), 10 mM α-benzyl-DL-serine and 0.1 mM pyridoxal phosphate was reacted at 30° C. for 10 minutes. After 10 minutes, the reaction was stopped by mixing 0.1 mL of an alkali reagent (5 N potassium hydroxide) attached in a formaldehyde kit, Test Wako (Wako Pure Chemicals Industries Ltd.).

出典