反応 #443752

反応方程式

N-formyl-L-aspartic acid

L-phenylalanine methyl ester

D,L-phenylalanine methyl ester

N-formyl-L-aspartic acid

D,L-phenylalanine methyl ester

→

N-formyl-α-L-aspartyl-L-phenylalanine methyl ester

溶媒

water

反応条件

後処理

1. 1
   その他the condensation reaction in the aqueous phase

実験手順

An enzymatic method for producing N-formyl-α-L-aspartyl-L-phenylalanine methyl ester by a condensation reaction between N-formyl-L-aspartic acid and L-phenylalanine methyl ester or D,L-phenylalanine methyl ester, which comprises: supplying an organic phase comprising a water-immiscible solvent containing N-formyl-L-aspartic acid and L- or D,L-phenylalanine methyl ester onto an aqueous phase comprising a thermolysin-like metalloprotease; proceeding the condensation reaction in the aqueous phase to produce N-formyl-α-L-aspartyl-L-phenylalanine methyl ester; and extracting the N-formyl-α-L-aspartyl-L-phenylalanine methyl ester thus produced from the aqueous phase to the organic phase.

出典