反応 #42631

ord-94874c96d452428c957b90a24f0b4475

反応方程式

O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21)NCCCCCC(=O)ON1C(=O)CCC1=O
NHS-LC-BIOTIN
O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21)NCCCCCC(=O)ON1C(=O)CCC1=O
NHS-LC-BIOTIN
O=C([O-])[O-]
carbonate
→
O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
biotin

反応物

O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21)NCCCCCC(=O)ON1C(=O)CCC1=O
NHS-LC-BIOTIN
O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21)NCCCCCC(=O)ON1C(=O)CCC1=O
NHS-LC-BIOTIN
O=C([O-])[O-]
carbonate

試薬

なし

反応条件

詳細条件
See reaction.notes.procedure_details.

後処理

  1. 1
    workup.STIRRINGAfter stirring
  2. 2
    その他this solution was dialyzed against physiological phosphate buffered saline (PBS)

実験手順

Anti-rabbit IgG monoclonal antibodies (2B9 5 μg/mL) dissolved in 0.1 M carbonate buffer (pH 8.5) and NHS-LC-BIOTIN (PIERCE, 25 μg/mL) were mixed and stirred at room temperature for four hours using a stirrer. IgG and NHS-LC-BIOTIN were mixed at a mole ratio of 1:60. After stirring, this solution was dialyzed against physiological phosphate buffered saline (PBS) to obtain biotin-labeled antibodies.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: US07732154B2uspto-grants-2010_06