反応 #42433

反応方程式

methyl 3-{[isopentyl(methyl)amino]methyl}-5-methylbenzoate

HATU

lithium hydroxide monohydrate

diisopropylethylamine

(2R,3S)-3-amino-4-(3,5-difluorophenyl)-1-{[1-(3-ethylphenyl)cyclopropyl]amino}-3-methylbutan-2-ol dihydrochloride

SP-211

→

title compound

N-((1S,2R)-1-(3,5-Difluorobenzyl)-3-{[1-(3-ethylphenyl)cyclopropyl]amino}-2-hydroxypropyl)-3-{[isopentyl(methyl)amino]methyl}-5-methylbenzamide dihydrochloride

溶媒

tetrahydrofuran methanol water

ethyl acetate

反応条件

後処理

1. 1
   濃縮The solution is concentrated under reduced pressure
2. 2
   workup.DISSOLUTIONredissolved in DMF (5 mL)
3. 3
   workup.ADDITIONare added
4. 4
   workup.STIRRINGThe reaction stirred at room temperature 2 h
5. 5
   洗浄washed with water, saturated sodium bicarbonate, brine
6. 6
   乾燥dried (sodium sulfate)
7. 7
   ろ過filtered
8. 8
   濃縮concentrated under reduced pressure
9. 9
   その他Purification by flash column chromatography (silica, 8% methanol/methylene chloride)

実験手順

To methyl 3-{[isopentyl(methyl)amino]methyl}-5-methylbenzoate prepared by the method in Example SP-211, Step 1 (160 mg, 0.61 mmol) in tetrahydrofuran/methanol/water (2:1:1, 8 mL) is added lithium hydroxide monohydrate (51 mg, 1.2 mmol), and the reaction is stirred at room temperature 16 h. The solution is concentrated under reduced pressure, redissolved in DMF (5 mL), and diisopropylethylamine (424 μL, 2.4 mmol), HATU (290 mg, 0.8 mmol), and (2R,3S)-3-amino-4-(3,5-difluorophenyl)-1-{[1-(3-ethylphenyl)cyclopropyl]amino}-3-methylbutan-2-ol dihydrochloride prepared by the method in Example SP-272 (291 mg, 0.7 mmol) are added. The reaction stirred at room temperature 2 h. The reaction mixture is diluted with ethyl acetate, washed with water, saturated sodium bicarbonate, brine, dried (sodium sulfate), filtered, and concentrated under reduced pressure. Purification by flash column chromatography (silica, 8% methanol/methylene chloride) provides the title compound as the free base. The residue is dissolved in diethyl ether (3 mL) and 1N hydrochloric acid in diethyl ether (2 mL) is added. The mixture is concentrated under reduced pressure to yield the title compound. ESI MS m/z 592.3 [M+H]+.

出典