反応 #41107

反応方程式

2-aminoadamantane hydrochloride

DIPEA

TSTU

2′-Methyl-2′H-[1,3′]bipyrazolyl-4′-carboxylic acid

Intermediate 8

2′-Methyl-2′H-[1,3′]bipyrazolyl-4′-carboxylic acid

→

2′-methyl-2′H-[1,3′]bipyrazolyl-4′-carboxylic acid adamantan-2-ylamide

収率 77.0%

溶媒

water

dichloromethane

DMF

反応条件

後処理

1. 1
   その他the organic layer was separated
2. 2
   抽出The aqueous layer was extracted twice with dichloromethane
3. 3
   その他The combined organic phases were dried under vacuum
4. 4
   その他purified by C-18

実験手順

2′-Methyl-2′H-[1,3′]bipyrazolyl-4′-carboxylic acid (Intermediate 8, 50 mg, 0.26 mmol) was dissolved in a mixture of dry dichloromethane (3.2 mL) and dry DMF (0.8 mL). DIPEA (0.23 mL, 1.3 mmol) and TSTU (93 mg, 0.28 mmol) were added to the above mixture. After the mixture was stirred for 1 h, the appearance of active ester was detected by LC-MS. Then 2-aminoadamantane hydrochloride (58 mg, 0.31 mmol) was added. After another 2 hours water was added and the organic layer was separated. The aqueous layer was extracted twice with dichloromethane. The combined organic phases were dried under vacuum and purified by C-18 reversed phase prep-HPLC with a gradient of 10-100% acetonitrile/water to give 2′-methyl-2′H-[1,3′]bipyrazolyl-4′-carboxylic acid adamantan-2-ylamide (65 mg, 77%). Mass spectrum: m/z: 362.2 (M+1).

出典