反応 #347434

ord-81ea2360901b4ab6ad533dc50ccdf0ca

反応方程式

CN1C(=O)CC[C@H]1c1cccnc1
cotinine
O=P(O)(O)OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O
glucose-6-phosphate
NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
NAD
→
CN1C(=O)CC[C@H]1c1cccnc1
Cotinine
NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1.O=P(O)(O)OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O
glucose-6-phosphate NAD

反応物

CN1C(=O)CC[C@H]1c1cccnc1
cotinine
O=P(O)(O)OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O
glucose-6-phosphate
NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
NAD

試薬

なし

反応条件

詳細条件
See reaction.notes.procedure_details.

後処理

  1. 1
    workup.ADDITIONdiluted 500-fold to a final concentration of 2.0 μg/ml

実験手順

Cotinine-enzyme conjugate was prepared at 1 mg/ml of protein and diluted 500-fold to a final concentration of 2.0 μg/ml. The anti-cotinine antibody was present in stock solution at an approximate concentration of 15 mg/ml. The glucose-6-phosphate-NAD solution was prepared by mixing 3 volumes of 0.11M glucose-6-phosphate with 2 volumes of 0.1M NAD.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: US05527686uspto-grants-1996_06