反応 #302885

反応方程式

2-(2-methyl-phenyl)-N-methyl-2-oxo-acetamide

NADPH

phosphate

IPTG

ampicillin

→

2-(2-methyl-phenyl)-N-methyl-2-hydroxy-acetamide

溶媒

ethyl acetate

反応条件

後処理

1. 1
   その他(37° C., 15 hours)
2. 2
   その他to obtain 0.1 g of wet bacterial cells
3. 3
   その他these were reacted at a stirring rate of 1000 rpm at 30° C. for 20 hours
4. 4
   workup.ADDITIONwas added to the reaction solution
5. 5
   その他(1000×g, 5 minutes)
6. 6
   その他to recover an organic layer
7. 7
   workup.DISTILLATIONThe organic layer was distilled off

実験手順

Using the plasmid pTrcGSRxc, E. coli HB101 was transformed. The resulting transformant was inoculated in a sterilized LB medium (100 ml) containing 0.1 mM of IPTG and 50 μg/ml of ampicillin, and was shaking-cultured (37° C., 15 hours). The resulting culture was centrifuged to obtain 0.1 g of wet bacterial cells. To a reaction tube were added 1.5 mg of 2-(2-methyl-phenyl)-N-methyl-2-oxo-acetamide, 0.1 g of the wet bacterial cells, 10 mg of NADPH, and 1.5 ml of a 100 mM phosphate buffer solution (pH 7), and these were reacted at a stirring rate of 1000 rpm at 30° C. for 20 hours. After the completion of the reaction, 5 ml of ethyl acetate was added to the reaction solution, and this was centrifuged (1000×g, 5 minutes) to recover an organic layer. The organic layer was distilled off to obtain oily 2-(2-methyl-phenyl)-N-methyl-2-hydroxy-acetamide. Content analysis by liquid chromatography under the following condition showed that the conversion rate was 87.2%. 1H-NMR analysis result of the resulting 2-(2-methyl-phenyl)-N-methyl-2-hydroxy-acetamide is shown below.

出典