反応 #217754

反応方程式

2,6-Diamino-9-(2-fluoro-β-D-arabinosyl)purine

adenosine

→

title compound

収率 100.0%

9-(2-Fluoro-β-D-arabinosyl)guanine

収率 100.0%

溶媒

Tris-HCl

反応条件

後処理

1. 1
   その他The resultant reaction mixture
2. 2
   ろ過was subjected to filtration by use of a membrane
3. 3
   ろ過filter (PTFE, 0.5 μm)
4. 4
   その他the filtrate was prepared into 60 mL of an aqueous solution
5. 5
   その他The aqueous solution was purified by means of reverse-phase ODS column chromatography (80 mL, 0 to 2% aqueous acetonitrile)

実験手順

2,6-Diamino-9-(2-fluoro-β-D-arabinosyl)purine (61.2 mg, 0.21 mmol) was dissolved in 50 mM Tris-HCl buffer solution (20 mL, pH 7.0), and adenosine deaminase (71 units) was added to the resultant solution, followed by stirring at room temperature for 1.5 hours. The resultant reaction mixture was subjected to filtration by use of a membrane filter (PTFE, 0.5 μm), and the filtrate was prepared into 60 mL of an aqueous solution. The aqueous solution was purified by means of reverse-phase ODS column chromatography (80 mL, 0 to 2% aqueous acetonitrile), to thereby yield 60.4 mg of the title compound as colorless crystals (yield: 100%).

出典