反応 #2028936

反応方程式

EDC

N-[(1S)-1-methyl-2-phenylethyl]butane-1,4-diamine

di-isopropylethylamine

→

Amphetamine

溶媒

water

dioxan

water

water

反応条件

後処理

1. 1
   workup.STIRRINGThe mixture was shaken for 18 hours at 20-25° C
2. 2
   その他The above material was dialysed against 50 mM Tris buffer at pH 7.4
3. 3
   workup.ADDITIONcontaining sodium azide using a 10,000 molecular weight
4. 4
   その他BCA protein assay after dialysis
5. 5
   その他gave a concentration of 1.6 mg/ml

実験手順

To BSA (24.8 mg) in water (4 ml) was added NHS (22.2 mg, 0.193 mmol) in water (0.5 ml) followed by EDC (41 mg, 0.199 mmol) in water (0.5 ml). The mixture was shaken for 15 minutes at 20-25° C. N-[(1S)-1-methyl-2-phenylethyl]butane-1,4-diamine (2.7 mg, 0.013 mmol) in dioxan (0.2 ml) followed by di-isopropylethylamine (0.02 ml). The mixture was shaken for 18 hours at 20-25° C. The above material was dialysed against 50 mM Tris buffer at pH 7.4 containing sodium azide using a 10,000 molecular weight cut off membrane. BCA protein assay after dialysis gave a concentration of 1.6 mg/ml.

出典