反応 #2006451

反応方程式

sodium hydroxide

N-Boc-3-pyrrolidinone

D-glucose

D-alanine

pyridoxal phosphate

N-Boc-3-pyrrolidinone

D-glucose

NAD+

D-alanine

pyridoxal phosphate

→

(R)—N-Boc-3-aminopyrrolidine

反応条件

後処理

1. 1
   その他(pNTASPAG), obtained in Example 11, which
2. 2
   workup.ADDITIONwas added so that total volume was 25 ml
3. 3
   workup.ADDITIONwere added

実験手順

To a flask containing 0.5 g of a substrate N-Boc-3-pyrrolidinone, 0.73 g of D-glucose, 4 mg of NAD+, 1.44 g of D-alanine, and 3.3 mg of pyridoxal phosphate, a culture fluid of the recombinant E. coli HB101 (pNTASPAG), obtained in Example 11, which expresses TAS, PALDH, and GDH was added so that total volume was 25 ml. The resultant product was stirred at 30° C. while being adjusted to a pH of 6.8 by drippage of 5 N aqueous solution of sodium hydroxide. After nine hours in reaction, 0.25 g of N-Boc-3-pyrrolidinone, 0.37 g of D-glucose, 0.37 g of D-alanine, and 3 mg of pyridoxal phosphate were added.

出典