反応 #1994273

反応方程式

( 3 )

5′-O-(tert-Butydimethylsilyl)-3′-O-methylthiomethyl-5-[3-(2,2,2-trifluoroacetamido)-prop-1-ynyl]-2′-deoxyuridine

cyclohexene

sulfurylchloride

N2

NaN3

→

( 4 )

収率 37.0%

3′-O-Azidomethyl-5-[3-(2,2,2-trifluoroacetamido)-prop-1-ynyl]-2′-deoxyuridine

収率 37.0%

溶媒

CH2Cl2

反応条件

後処理

1. 1
   その他the full consumption of the nucleoside (3)
2. 2
   その他The solvent was evaporated
3. 3
   workup.WAITthe residue was subjected to high vacuum for 20 min
4. 4
   workup.DISSOLUTIONIt was then redissolved in dry DMF (3 ml)
5. 5
   その他The reaction was quenched with CH2Cl2
6. 6
   洗浄the organic layers were washed with sat aq. NaCl solution
7. 7
   その他After removing the solvent
8. 8
   workup.DISSOLUTIONthe resulting yellow gum was redissolved in THF (2 ml)
9. 9
   workup.ADDITIONtreated with TBAF (1M in THF, 0.5 ml) at room temperature for 30 min
10. 10
    その他The solvent was removed
11. 11
    抽出The aqueous layer was extracted three times with CH2Cl2
12. 12
    その他Purification by chromatography on silica (EtOAc: petroleum ether 1:1 to EtOAc)

実験手順

To a solution of (3) (348 mg, 0.63 mmol) and cyclohexene (0.32 ml, 3.2 mmol) in dry CH2Cl2 (5 ml) at 4° C., sulfurylchloride (1M in CH2Cl2, 0.76 ml, 0.76 mmol) was added drop wise under N2. After 10 min TLC indicated the full consumption of the nucleoside (3). The solvent was evaporated and the residue was subjected to high vacuum for 20 min. It was then redissolved in dry DMF (3 ml) and treated with NaN3 (205 mg, 3.15 mmol). The resulting suspension was stirred under room temperature for 2 h. The reaction was quenched with CH2Cl2 and the organic layers were washed with sat aq. NaCl solution. After removing the solvent, the resulting yellow gum was redissolved in THF (2 ml) and treated with TBAF (1M in THF, 0.5 ml) at room temperature for 30 min. The solvent was removed and the reaction worked up with CH2Cl2 and sat. aq. NaHCO3 solution. The aqueous layer was extracted three times with CH2Cl2. Purification by chromatography on silica (EtOAc: petroleum ether 1:1 to EtOAc) gave (4) (100 mg, 37%) as a pale yellow foam. 1H NMR (d6 DMSO) δ 2.15-2.26 (m, 2H, H-2′), 3.47-3.57 (m, 2H, H-5′), 3.88-3.90 (m, 1H, H-4′), 4.14 (d, J=4.7 Hz, 2H, CH2NH), 4.24-4.27 (m, 1H, H-3′), 4.75 (s, 2H, CH2N3), 5.14 (t, J=5.2 Hz, 1H, OH), 5.96-6.00 (m, 1H, H-1′), 8.10 (s, 1H, H-6), 10.00 (s, 1H, NHCOCF3)), 11.26 (s, 1H, NH).

出典