反応 #1842392

ord-05dd12aaed8c491d922b32edbd1a7e07

反応方程式

O=C(CO)[C@@H](O)[C@H](O)[C@H](O)COP(=O)(O)O
fructose 6-phosphate
NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
NADH
O=C[C@H](O)[C@H](O)COP(=O)(O)O
erythrose 4-phosphate
→
O=C(CO)[C@@H](O)[C@H](O)[C@H](O)CO
Fructose

反応物

O=C(CO)[C@@H](O)[C@H](O)[C@H](O)COP(=O)(O)O
fructose 6-phosphate
NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
NADH
O=C[C@H](O)[C@H](O)COP(=O)(O)O
erythrose 4-phosphate

試薬

なし

溶媒

Cl.NC(CO)(CO)CO
Tris HCl
Cl.NC(CO)(CO)CO
Tris HCl

反応条件

詳細条件
See reaction.notes.procedure_details.

後処理

  1. 1
    その他The reaction for determination of transaldolase
  2. 2
    抽出extract

実験手順

Cell disruption was performed in Tris/HCl buffer (100 mM, pH 7.8). The reaction for determination of transaldolase was performed in 50 mM Tris/HCl buffer (pH 7.8) additionally containing 1 mM fructose 6-phosphate, 0.5 mM NADH, 4 mM erythrose 4-phosphate, 6 U TPI/GDH and 50 μl of crude cell extract. TPI/GDH served as coupling enzymes to allow online monitoring at 340 nm.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: US09169502B2uspto-grants-2015_10