反応 #1836023

ord-1b26caafa6a644a48874c7472e44e884

反応方程式

O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21)NCCCCCC(=O)ON1C(=O)CC(S(=O)(=O)[O-])C1=O.[Na+]
sulfo-NHS-LC-biotin
O=C([O-])[O-].[Na+].[Na+]
sodium carbonate
→
O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
biotin

反応物

O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21)NCCCCCC(=O)ON1C(=O)CC(S(=O)(=O)[O-])C1=O.[Na+]
sulfo-NHS-LC-biotin
O=C([O-])[O-].[Na+].[Na+]
sodium carbonate

試薬

なし

反応条件

詳細条件
See reaction.notes.procedure_details.

後処理

  1. 1
    その他The RA-NAH (10 mg) obtained in the
  2. 2
    その他at 4° C
  3. 3
    その他After overnight dialysis
  4. 4
    workup.DISTILLATIONagainst distilled water

実験手順

The RA-NAH (10 mg) obtained in the above was dissolved in 0.5 ml of 0.1 M sodium carbonate aqueous solution, subsequently adding 3 mg of sulfo-NHS-LC-biotin (mfd, by Pierce Chemical Company) thereto followed by overnight incubation at 4° C. After overnight dialysis against distilled water thereafter, the dialyzed liquid was lyophilized to obtain the polysaccharide of the present invention in which biotin is bound to the reducing end of NAH (to be referred to as “NAH-biotin” hereinafter) (8 mg).

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: US09150902B2uspto-grants-2015_10