反応 #1835894

ord-55c83ba394024cfdb19a1a24bbb667c8

反応方程式

O=C(O)/C=C/c1cnc[nH]1
urocanate
O=P([O-])([O-])OP(=O)([O-])[O-]
pyrophosphate
N[C@@H](CCC(=O)N[C@@H](CS)C(=O)NCC(=O)O)C(=O)O
glutathione
→
N.N[C@@H](Cc1c[nH]cn1)C(=O)O
Histidine Ammonia

反応物

O=C(O)/C=C/c1cnc[nH]1
urocanate
O=P([O-])([O-])OP(=O)([O-])[O-]
pyrophosphate
N[C@@H](CCC(=O)N[C@@H](CS)C(=O)NCC(=O)O)C(=O)O
glutathione

試薬

[Cl-].[Cl-].[Zn+2]
ZnCl2

溶媒

N[C@@H](Cc1c[nH]cn1)C(=O)O
histidine

反応条件

詳細条件
See reaction.notes.procedure_details.

後処理

  1. 1
    workup.WAITmonitored for approximately 5 minutes

実験手順

HAL enzyme activity can be determined as described in Baedeker & Schulz (Eur. J. Biochem 2002, 269, 1790-1797), wherein enzyme activity was determined as the rate of urocanate formation, measured spectrophotometrically at 277 nm. For a standard assay, the enzyme was preincubated at 25° C. for 5 min in 2.5 mL buffer containing 0.1 M pyrophosphate (pH 9.3), 10 μM ZnCl2 and 2 mM glutathione. The reaction was started by adding 200 μL of 0.5 M histidine solution and then monitored for approximately 5 minutes.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: US09150845B2uspto-grants-2015_10