反応 #1810793

反応方程式

Racemic ethyl 3-hydroxybutyrate

potassium phosphate

→

Ethyl (S)-3-hydroxybutyrate

収率 97.0%

反応条件

後処理

1. 1
   抽出The reaction mixture was extracted with ethyl acetate

実験手順

Racemic ethyl 3-hydroxybutyrate (1%, v/v) was added to the vial containing 5 ml potassium phosphate buffer (pH 8.0, 0.1 M) and Novozyme 435 (4%, w/v). The reaction was carried out at 30° C. for 2 hours. The reaction mixture was extracted with ethyl acetate and analyzed by above-mentioned method. Ethyl (S)-3-hydroxybutyrate (97% e.e) was obtained from organic solvent at 55% conversion. The aqueous solution was acidified with hydrochloric acid and extracted with organic solvent. After esterification, ethyl (R)-3-hydroxybutyrate with optical purity of 80.4% e.e was obtained.

出典