反応 #1755446

反応方程式

glyoxylic acid

L-cystine

borate

→

N,N,N′,N′-tetrakis(carboxymethyl)-L-cystine

反応条件

後処理

1. 1
   その他The alternative route, relying on in-solution phase synthesis of an intermediate product
2. 2
   その他purified with ion exchange chromatography

実験手順

The alternative route, relying on in-solution phase synthesis of an intermediate product starts with addition of a large excess (40 times) of glyoxylic acid to L-cystine in an alkaline borate buffer. The intermediate product, following pH manipulation and conductivity adjustment of the reaction mixture, purified with ion exchange chromatography to give N,N,N′,N′-tetrakis(carboxymethyl)-L-cystine.

出典