反応 #1702031

反応方程式

topotecan hydrochloride

sodium

cysteic acid

→

topotecan

溶媒

Methanol

反応条件

後処理

1. 1
   その他evaporated in vacuo
2. 2
   workup.ADDITION120 ml sodium chloride solution (9 mg/ml) was added to the residue
3. 3
   その他obtained
4. 4
   その他after evaporation
5. 5
   その他transparent (approx. 20 min)
6. 6
   その他The solution obtained
7. 7
   ろ過was filtered through 0.2 mm
8. 8
   ろ過filter
9. 9
   ろ過The filtration

実験手順

Methanol stock-solutions of topotecan hydrochloride (120 ml, 1.09 mg/ml) and sodium salt of methyl ester of N-all-trans-retinoyl cysteic acid (32 ml, 15 mg/ml) were mixed in a 500 ml round-bottom flask and evaporated in vacuo. 120 ml sodium chloride solution (9 mg/ml) was added to the residue obtained after evaporation, and the mixture was stirred until it became clear and transparent (approx. 20 min). The concentration of topotecan in the obtained solution was 1 mg/ml, corresponding to a topotecan hydrochloride concentration of 1.09 mg/ml. The solution obtained was filtered through 0.2 mm filter. The filtration did not result in reduction of topotecan concentration.

出典