反応 #1528389

ord-7ecdaa8541524bb59512d24ab4b045aa

反応方程式

CN(CC(=O)O)C(=N)N
creatine
Nc1nc2c(ncn2[C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]2O)c(=O)[nH]1
GTP
CN(CC(=O)O)C(=N)N.O=P(O)(O)O
creatine phosphate
CC(C)Cn1c(=O)n(C)c(=O)c2[nH]cnc21
IBMX
Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ATP
→
Nc1ncnc2c1ncn2[C@@H]1O[C@@H]2COP(=O)(O)O[C@H]2[C@H]1O
cAMP

反応物

—
Mg
CN(CC(=O)O)C(=N)N
creatine
Nc1nc2c(ncn2[C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]2O)c(=O)[nH]1
GTP
CN(CC(=O)O)C(=N)N.O=P(O)(O)O
creatine phosphate
CC(C)Cn1c(=O)n(C)c(=O)c2[nH]cnc21
IBMX
Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ATP

試薬

なし

溶媒

NC(CO)(CO)CO
Tris

反応条件

詳細条件
See reaction.notes.procedure_details.

後処理

  1. 1
    その他at 32° C
  2. 2
    workup.ADDITIONProspective ligands are added

実験手順

Membranes are incubated in 25 mM Tris (pH 7.6), 0.2% BSA, 2.6 mM Mg, 0.8 mM ATP, 0.1 mM GTP, 5 mM creatine phosphate, 50 U/ml creatine kinase, 0.2 mM IBMX at 32° C. Prospective ligands are added and incubation is continued for another 15 minutes. The amount of cAMP produced is assayed using an fluorescent immunoassay method.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: US07655396B1uspto-grants-2010_02