反応 #1375841

ord-2992097d8fa04bfe89306840efb827e3

反応方程式

OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O
maltose
→
OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O.OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O
maltose trehalose
OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O
maltose

反応物

OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O
maltose

試薬

なし

溶媒

O
water

反応条件

詳細条件
See reaction.notes.procedure_details.

後処理

  1. 1
    workup.ADDITIONadded at 15° C.

実験手順

Ten parts by weight of maltose commercialized by Hayashibara Biochemical Laboratories, Inc., Okayama, Japan, was dissolved in 40 parts by weight of water, added at 15° C. and pH7.0 to 2 units/g maltose solid of a purified maltose/trehalose conversion enzyme obtained by the method in Experiment 2, reacted for 48 hours and heated at 100° C. for 10 minutes to inactivate the enzyme. The resultant solution contained about 74% trehalose on dry solid basis. The solution was then decolored with activated carbon, deionized and purified with ion exchanges of H- and OH-forms, concentrated to about 78%, added with 0.1% crystalline trehalose hydrate as seed crystal on dry solid basis and allowed to standing at ambient temperature overnight to effect crystallization. The resultant massecuite was separated and the crystals were sprayed with a minimum amount of water for washing, thus obtaining about 3.0 parts by weight of an extremely pure crystalline trehalose hydrate, purity of 99.8%.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: US05747300uspto-grants-1998_05