反応 #1265349

反応方程式

histidine

sodium chloride

→

histidine sodium chloride

溶媒

water

反応条件

後処理

1. 1
   その他Ethanol was removed from the liposome suspension by diafiltration
2. 2
   ろ過The solution was filtered through a 0.22 μm
3. 3
   ろ過Durapore filter
4. 4
   workup.ADDITIONThe liposome suspension was diluted in approximately a 1:1 (v/v) ratio with the histidine/sodium chloride solution
5. 5
   その他to remove the ethanol

実験手順

Ethanol was removed from the liposome suspension by diafiltration. A histidine/sodium chloride solution was prepared by dissolving histidine (10 mM) and sodium chloride (150 mM) in sterile water. The pH of the solution was adjusted to approximately 7. The solution was filtered through a 0.22 μm Durapore filter. The liposome suspension was diluted in approximately a 1:1 (v/v) ratio with the histidine/sodium chloride solution and diafiltered through a polysulfone hollow-fiber ultrafilter. Eight volume exchanges were performed against the histidine/sodium chloride solution to remove the ethanol. The process fluid temperature was maintained at about 20-30° C. Total diafiltration time was approximately 4.5 hours.

出典