反応 #1244242

ord-a8157e506ba647088ae91dc2f0088eb6

反応方程式

O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate
C=C(OP(=O)(O)O)C(=O)O
phosphoenolpyruvate
N[C@@H](CCC(=O)[O-])C(=O)[O-]
glutamate
→
N[C@@H](CCC(=O)O)C(=O)O
L-glutamic Acid

反応物

O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate
C=C(OP(=O)(O)O)C(=O)O
phosphoenolpyruvate
N[C@@H](CCC(=O)[O-])C(=O)[O-]
glutamate

試薬

なし

反応条件

詳細条件
See reaction.notes.procedure_details.

後処理

  1. 1
    workup.ADDITIONwere introduced into the SC17sucA strain

実験手順

Subsequently, the citrate synthase gene, phosphoenolpyruvate carboxylase gene and glutamate dehydrogenase gene derived from Escherichia coli were introduced into the SC17sucA strain.

出典

DOI: 10.6084/m9.figshare.5104873.v1特許: USRE041800E1uspto-grants-2010_10