反応 #1178409

反応方程式

sodium formate

acetophenone

→

(S)-phenylethanol

収率 96.4%

溶媒

formic acid

反応条件

後処理

1. 1
   その他Culture fluid was obtained
2. 2
   workup.ADDITIONwas added dropwise to the mixture
3. 3
   その他After the reaction
4. 4
   抽出the reaction mixture was extracted with toluene
5. 5
   乾燥was dried over anhydrous sodium sulfate
6. 6
   その他Removal of sodium sulfate, and evaporation of organic solvents from the organic layer under reduced pressure

実験手順

Culture fluid was obtained by cultivating E. coli HB101 (pNCMFT) in a similar manner to Example 9. In a 100-mL portion of the culture fluid, 2.8 g of sodium formate, 3 mg of NAD+, and 10 g of acetophenone were added, and the mixture was stirred at 30° C. for 20 hours while controlling the pH of the reaction mixture to 6.0 with 5 N aqueous formic acid, which was added dropwise to the mixture. After the reaction, the reaction mixture was extracted with toluene, and combined organic layer was dried over anhydrous sodium sulfate. Removal of sodium sulfate, and evaporation of organic solvents from the organic layer under reduced pressure yielded 9.8 g of (S)-phenylethanol. The product (S)-phenylethanol was analyzed in accordance with the [Analysis condition (1) for gas chromatography] as described above, and found that the optical purity of the product was 99.9% e.e. or higher.

出典