反応 #1148662
ord-d6f5b387ebd944f0835dcb70f656f92a
反応条件
後処理
- 1その他to react for at least 24 hours at room temperature
- 2その他To separate fluorescent-Desferrioxamine from free fluorescein and free Desferrioxamine
- 3洗浄are eluted in 2 ml fractions
- 4洗浄The fluorescein-Desferrioxamine is eluted in the void volume of the column
- 5その他The first peak is collected
- 6その他used for preparing fluorescein-Desferrioxamine, Desferrioxamine, and rhodamine-liposomes
実験手順
An aliquot of 120 mg of Desferrioxamine and 233.64 mg of fluorescein isothiocyanate (FITC) are mixed in 50 ml of 0.1 M Na carvonate pH 9.5. A molar ratio of 3:1 of FITC: Desferrioxamine is used as described in Hentz et al. (1997) Anal. Chem. 69:4994-5000. The reaction mixture is wrapped in aluminum foil to avoid light exposure and allowed to react for at least 24 hours at room temperature. To separate fluorescent-Desferrioxamine from free fluorescein and free Desferrioxamine, the mixture is electrophoresed over a P-2 column (Bio-Rad) equilibrated in saline and samples are eluted in 2 ml fractions. The fluorescein-Desferrioxamine is eluted in the void volume of the column. The first peak is collected and used for preparing fluorescein-Desferrioxamine, Desferrioxamine, and rhodamine-liposomes.