反応 #1121749

反応方程式

NaOH

NaOH

methanol

5-methyl-3-nitromethyl-hexanoic acid ethyl ester

(S)-5-methyl-3-nitromethyl-hexanoic acid ethyl ester

→

title compound

(S)-5-methyl-3-nitromethyl-hexanoic acid sodium salt

溶媒

potassium phosphate

反応条件

後処理

1. 1
   その他After 45% conversion (corresponding to 4.0 mL of 0.1M NaOH consumption) the reaction
2. 2
   workup.ADDITIONwas stopped by addition of 10 mL of ethyl acetate
3. 3
   その他The layers were separated
4. 4
   抽出the aqueous layer was extracted once more with 10 mL of ethyl acetate
5. 5
   濃縮Then the aqueous layer was concentrated

実験手順

In a beaker 100 mg of EstC (lyophilized) were dissolved/suspended in 10 mL of potassium phosphate buffer (1 mM, pH 7.2). The pH drops to pH ˜6.8 and was adjusted to pH=7.4 with aqueous NaOH (0.1 M). Then 250 μL of methanol and 200 mg of 5-methyl-3-nitromethyl-hexanoic acid ethyl ester (VIII, R1=ethyl) were added and the pH was kept at 7.4 by continuous addition of aqueous NaOH (0.1M). After 45% conversion (corresponding to 4.0 mL of 0.1M NaOH consumption) the reaction was stopped by addition of 10 mL of ethyl acetate. The layers were separated and the aqueous layer was extracted once more with 10 mL of ethyl acetate. Then the aqueous layer was concentrated to give the title compound with an ee of 98%.

出典