Réaction #8957

Équation de réaction

L-arginine methyl ester dihydrochloride

sodium hydroxide

→

L-arginine

Solvants

methanol

Conditions de réaction

Traitement

1. 1
   Autreto prepare a treating agent solution
2. 2
   Autreto uniformly absorb the total amount of the above treating agent solution
3. 3
   Autreair-dried for an hour at room temperature
4. 4
   workup.ADDITIONThe dried cloth was heat-treated for 20 minutes at 140° C. in a batch type hot air oven (“IPHH-2000”, an inert oven manufactured by Tabaiesbeck Co., Ltd.)
5. 5
   Lavagewashed with water
6. 6
   LavageIt was further washed with 5% aqueous sodium bicarbonate solution
7. 7
   Lavagerinsed three times with water
8. 8
   Lavagesubsequently washed with 10% aqueous citric acid solution
9. 9
   Lavagerinsed three times with water
10. 10
    Lavagefinally washed with a JAFET standard detergent solution for household
11. 11
    Lavagewashing machine
12. 12
    Lavagerinsed with water
13. 13
    Autreair-dried

Mode opératoire

1.31 Gram (5 mmol) of L-arginine methyl ester dihydrochloride was dissolved in 4 ml of methanol, and 2.5 ml of 2 N aqueous sodium hydroxide solution was added thereto to prepare a treating agent solution. An untreated cotton cloth (160 mm×170 mm, 5.0 g) was allowed to uniformly absorb the total amount of the above treating agent solution and then air-dried for an hour at room temperature. The dried cloth was heat-treated for 20 minutes at 140° C. in a batch type hot air oven (“IPHH-2000”, an inert oven manufactured by Tabaiesbeck Co., Ltd.) and then washed with water. It was further washed with 5% aqueous sodium bicarbonate solution and rinsed three times with water and subsequently washed with 10% aqueous citric acid solution and rinsed three times with water, and finally washed with a JAFET standard detergent solution for household washing machine, rinsed with water and air-dried. A part of this cloth was cut and a solution of ninhydrin in butanol was sprayed on it. Thereafter, it was heated for 10 minutes in an oven of 110° C. to cause coloring thereby the presence of the amino acid was confirmed. As a result of determination for the amount of the bonded L-arginine, it was 0.08 mmol based on 1 g of the cloth. The degree of esterfication was 0.013.

Source