Réaction #6343

Équation de réaction

6-[3-benzyloxy-2-(diethoxyphosphorylmethoxy)propoxyamino]-4-chloro-2,5-diformamidopyrimidine

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title compound

Rendement 75.0%

9-[3-Benzyloxy-2-(diethoxyphosphorylmethoxy)propoxy]-6-chloro-2-formamidopurine

Rendement 75.0%

Solvants

diethoxymethyl acetate

Conditions de réaction

Traitement

1. 1
   TempératureAfter cooling to ambient temperature
2. 2
   Autrethe solvent was evaporated
3. 3
   workup.DISSOLUTIONthe residue dissolved in methanol (10 ml)
4. 4
   AutreAfter 15 minutes at ambient temperature the solution was evaporated to dryness
5. 5
   AutreThe residue obtained
6. 6
   Autrewas chromatographed on silica gel (dichloromethane/methanol, 98:2 as eluant)

Mode opératoire

A solution of 6-[3-benzyloxy-2-(diethoxyphosphorylmethoxy)propoxyamino]-4-chloro-2,5-diformamidopyrimidine (760 mg, 1.4 mmol) in diethoxymethyl acetate (2 ml) was stirred and heated in an oil bath at 120° C. for 2 hours. After cooling to ambient temperature, the solvent was evaporated and the residue dissolved in methanol (10 ml) and 0.880 ammonia (1 ml). After 15 minutes at ambient temperature the solution was evaporated to dryness. The residue obtained was chromatographed on silica gel (dichloromethane/methanol, 98:2 as eluant) to give the title compound as a colourless oil (550 mg, 75%). IR: νmax (film), 3400, 3220, 3110, 2980, 2900, 1710, 1610, 1580, 1510, 1475, 1440, 1385, 1330, 1240, 1160, 1140, 1095, 1050, 1020, 980, 920, 820, 780, 740, 700 cm-1. 1H NMR: δH [(CD3)2SO] 1.20, 1.21 (6H, 2×t, P-(OCH2CH3)2), 3.6-3.75 (2 H, m, CHCH2), 3.8-4.15 (7H, m, CH2CHCH2, OCH2P, P-(OCH2CH3)2), 4.52 (2H, s, OCH2Ph), 4.45-4.65 (2H, m, N-OCH2), 7.25-7.40 (5H, m, Ph), 8.81 (1H, s, H-8), 9.37 (1H, br.s, NHCHO), 11.31 (1H, br.s, D2O exchangeable NHCHO). Found: C, 48.11; H, 5 37; N, 12.50%. C21H27N5O7PCl requires: C, 47.77; H, 5.16; N, 13.27%. m/z: C21H28N5O7PCl requires 528.1415; observed 528.1363 (M +H+).

Source