Réaction #5857

ord-f252bc5bba1b49918808b53ec81978dd

Équation de réaction

Cc1c(N)c(=O)n(-c2ccccc2)n1C
4-aminoantipyrine
N.O=c1ccn(C2OC(COP(=O)(O)OC3C(CO)OC(n4ccc(=O)[nH]c4=O)C3O)C(O)C2O)c(=O)[nH]1
uricase
→
O=c1[nH]c(=O)c2[nH]c(=O)[nH]c2[nH]1
Uric Acid

Réactifs

Cc1c(N)c(=O)n(-c2ccccc2)n1C
4-aminoantipyrine
N.O=c1ccn(C2OC(COP(=O)(O)OC3C(CO)OC(n4ccc(=O)[nH]c4=O)C3O)C(O)C2O)c(=O)[nH]1
uricase

Réactifs

Aucun

Solvants

O=P([O-])([O-])[O-]
phosphate

Conditions de réaction

Température
37°CELSIUS
Conditions détaillées
See reaction.notes.procedure_details.

Traitement

  1. 1
    workup.ADDITIONTo each of the mixtures was added a reagent
  2. 2
    Autreprepared
  3. 3
    Autreusing purified water as a control
  4. 4
    AutreThe results obtained

Mode opératoire

To each of the mixtures was added a reagent prepared by dissolving 1 μmol 4-aminoantipyrine and 2 units of uricase in 1.5 ml of 0.1M phosphate buffer (pH 6.5). After the resulting mixtures were maintained in a thermostat at 37° C. for about 5 minutes, the absorbance was measured at a wavelength of 555 nm with a spectrophotometer (Model 228, manufactured by Hitachi, Ltd.) using purified water as a control. The results obtained are shown in Table 3.

Source

DOI: 10.6084/m9.figshare.5104873.v1Brevet: US05246836uspto-grants-1993_09