Réaction #5238

Équation de réaction

[3H]spiperone

(+)-butaclamol

Tris HCl

NaCl

MgCl2

→

Dopamine

Conditions de réaction

Traitement

1. 1
   Autre0.5 mL aliquots of the membrane preparation
2. 2
   Filtrationsamples were filtered rapidly through Whatman GF/C glass filters under negative pressure
3. 3
   Lavagewashed three times with ice-cold binding buffer (5 mL)

Mode opératoire

0.5 mL aliquots of the membrane preparation were incubated with unlabeled drugs, and 0.15 nM [3H]spiperone in a final volume of 1 mL containing 50 mM Tris HCl, 120 mM NaCl and 1 mM MgCl2 (pH 7.7). Nonspecific binding was measured in the presence of 100 nM (+)-butaclamol. After 15 minutes of incubation at 37° C., samples were filtered rapidly through Whatman GF/C glass filters under negative pressure, and washed three times with ice-cold binding buffer (5 mL).

Source