Réaction #470404

ord-7dfc20e410c4461792a3ef6bd70e3bcb

Équation de réaction

O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
O=C[C@H](O)[C@@H](O)[C@H](O)CO
xylose
→
OC[C@@H](O)[C@H](O)[C@@H](O)CO
xylitol

Réactifs

O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
O=C[C@H](O)[C@@H](O)[C@H](O)CO
xylose

Réactifs

Aucun

Solvants

CCO
ethanol

Conditions de réaction

Conditions détaillées
See reaction.notes.procedure_details.

Traitement

  1. 1
    Autrexylose consumption, ethanol concentration and product
  2. 2
    Autreyields after 117 hours of fermentation
  3. 3
    AutreThe reference strain Y-PsNative consumed 30.4 g l−1 xylose
  4. 4
    Autreproduced 16.7 g l−1 ethanol
  5. 5
    Autrewhile Y-PsK270R consumed 46.1 g l−1 xylose
  6. 6
    Autreproduced 25.3 g l−1 ethanol
  7. 7
    AutreY-PsK270M consumed the least xylose (16.8 g l−1)
  8. 8
    Autreproduced
  9. 9
    Concentrationthe lowest ethanol concentration (14.1 g l−1) of the three strains
  10. 10
    AutreThe reference strain Y-PsNative produced an ethanol yield of 0.33 g ethanol
  11. 11
    Autreg consumed sugars−1
  12. 12
    Autrea xylitol yield of 0.26 g xylitol g consumed xylose−1
  13. 13
    AutreBoth strains with mutated XR produced higher ethanol

Mode opératoire

Strains Y-PsNative, Y-PsK270M and Y-PsK270R (cf example 4) were compared in anaerobic batch fermentation with 20 g l−1 glucose and 50 g l−1 xylose (FIG. 3). Table 6 summarizes xylose consumption, ethanol concentration and product yields after 117 hours of fermentation. The reference strain Y-PsNative consumed 30.4 g l−1 xylose and produced 16.7 g l−1 ethanol while Y-PsK270R consumed 46.1 g l−1 xylose and produced 25.3 g l−1 ethanol. Y-PsK270M consumed the least xylose (16.8 g l−1) and produced the lowest ethanol concentration (14.1 g l−1) of the three strains. The reference strain Y-PsNative produced an ethanol yield of 0.33 g ethanol g consumed sugars−1 and a xylitol yield of 0.26 g xylitol g consumed xylose−1. Both strains with mutated XR produced higher ethanol yields (0.38 g ethanol (g consumed sugars)−1) and significantly lower xylitol yields (0.09 g xylitol (g consumed xylose)−1) than the reference strain. In addition, Y-PsK270R grew and produced biomass anaerobically from xylose after glucose depletion (FIG. 4). Y-PsK270R had produced 3.4 g l−1 biomass after 117 hours of fermentation while both Y-PsNative and Y-PsK270M produced 2.1 g l−1 biomass.

Source

DOI: 10.6084/m9.figshare.5104873.v1Brevet: US08367393B2uspto-grants-2013_02