Réaction #4260

Équation de réaction

FITC

carbonate

Fluoresceinisothiocyanate

→

Fluorescein

Solvants

ethanol

Conditions de réaction

Traitement

1. 1
   Autrethe free FITC is chromatographically separated from the bound molecules on a Sephadex G50 column
2. 2
   AutreThe labeled protein is collected in the void volume

Mode opératoire

Fluoresceinisothiocyanate (FITC) is dissolved in ethanol (5 mg solid/ml). To 2 ml of a 5 mg/ml protein solution from (I), 0.5 ml carbonate buffer (1M NaHCO3 --Na2CO3 buffer pH 9) is added, followed by 50 μl FITC solution. The mixture is shaken well and the free FITC is chromatographically separated from the bound molecules on a Sephadex G50 column using a buffer comprising 10 mM Tris, 1 mM EDTA, 50 mM KCl, pH 7.4. The labeled protein is collected in the void volume.

Source