Réaction #358223

ord-d18ca109c7f44033868fc43d034e8562

Équation de réaction

[Na+].[OH-]
sodium hydroxide
O=C([O-])C(=O)Cc1ccccc1.[Na+]
sodium phenylpyruvate
NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](OP(=O)(O)O)[C@@H]3O)[C@@H](O)[C@H]2O)c1
NADP
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
CN.Cl
methylamine hydrochloric acid
→
CN[C@@H](Cc1ccccc1)C(=O)O
N-methylphenylalanine
Rendement 75.0%

Réactifs

[Na+].[OH-]
sodium hydroxide
O=C([O-])C(=O)Cc1ccccc1.[Na+]
sodium phenylpyruvate
NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](OP(=O)(O)O)[C@@H]3O)[C@@H](O)[C@H]2O)c1
NADP
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
CN.Cl
methylamine hydrochloric acid
—
20

Réactifs

Aucun

Conditions de réaction

Température
30°CELSIUS
Conditions détaillées
See reaction.notes.procedure_details.

Traitement

  1. 1
    AutreTo the resting cells obtained
  2. 2
    workup.ADDITIONwas added

Mode opératoire

To the resting cells obtained, a reaction solution containing sodium phenylpyruvate in a final concentration of 100 mM, NADP in a final concentration of 0.2 mM, glucose in a final concentration of 100 mM, and methylamine-hydrochloric acid (pH 9) in a final concentration of 700 mM was added to achieve cell turbidity of 20 (absorbance at 660 nm). The reaction was conducted while stirring at 30° C. and adjusting pH to 8 to 9 with 10 N aqueous sodium hydroxide solution. The amount of N-methylphenylalanine produced after 24 hours of reaction was 13.4 g/L (reaction yield=75%).

Source

DOI: 10.6084/m9.figshare.5104873.v1Brevet: US07452704B2uspto-grants-2008_11