Réaction #350570

Équation de réaction

NADP+

MgCl2

glucose 1,6-diphosphate

glucose-6-phosphate

potassium phosphate

trehalose

glutathione

→

NADPH

Solvants

Conditions de réaction

Traitement

1. 1
   Autreis incubated at 30° C.

Mode opératoire

The activity of the enzyme is determined by the following procedure. That is, a total 100 ml buffer solution of 57 mM potassium phosphate containing 10.8 g of trehalose, 860 mg of glutathione and 17.2 mg of EDTA·2Na, pH 7.0, is prepared. A mixture of 1,400 μl of the buffer solution, 100 μl of 20 mM NADP+ aqueous solution, 100 μl of 26 mM MgCl2 aqueous solution, 100 μl of 1.34 mM glucose 1,6-diphosphate aqueous solution, 100 μl of 31 U/ml phosphoglucomutase aqueous solution, 100 μl of 35 U/ml glucose-6-phosphate dehydrogenase aqueous solution and 100 μl of a test sample enzyme solution is incubated at 30° C. and the amount of NADPH produced is determined by the absorbance at 340 nm with the passage of lime. The quantity of enzyme which produces one μ mole of NADPH in one minute under the above reaction conditions is made one Unit.

Source