Réaction #319691

ord-d0f7a5dd9a9e4a499ca06ed3a43f1697

Équation de réaction

O=P([O-])([O-])[O-]
phosphate
N=C(N)NCCC[C@H](N)C(=O)O
arginine
O=C1O[C@H]([C@@H](O)CO)C(O)=C1O
ascorbic acid
N=C(N)NCCCC(N)C(=O)O
DL-arginine
→
NCCC[C@H](N)C(=O)O
ornithine

Réactifs

O=P([O-])([O-])[O-]
phosphate
N=C(N)NCCC[C@H](N)C(=O)O
arginine
O=C1O[C@H]([C@@H](O)CO)C(O)=C1O
ascorbic acid
N=C(N)NCCCC(N)C(=O)O
DL-arginine

Réactifs

O=S(=O)([O-])[O-].[Mn+2]
manganese sulfate

Solvants

O
H2O
CC#N
acetonitrile
O
water

Conditions de réaction

Conditions détaillées
See reaction.notes.procedure_details.

Mode opératoire

1.74 g DL-arginine (50:50) were dissolved in 10 ml water and mixed with 0.21 mg manganese sulfate.H2O, 0.11 mg ascorbic acid and 0.5 mg calf liver L-arginase (Boeringer Mannheim, 97 U/mg. The progress of the reaction was followed with HPLC (amino column; mobile solvent 65:35 acetonitrile:phosphate buffer (pH 6.4). After 6 hours at 25° C., 50% of the original amount of arginine had reacted and a corresponding amount of ornithine had been formed (conversion 100%).

Source

DOI: 10.6084/m9.figshare.5104873.v1Brevet: US05591613uspto-grants-1997_01