Réaction #302909

Équation de réaction

13

1-(5-O-Acetyl-2-deoxy-3-O-methanesulfonyl-4-ethynyl-β-D-threo-pentofuranosyl)thymine

DBN

MeOH

→

2′,3′-didehydro-3′-deoxy-4′-ethynylthymidine

Solvants

CH3CN

Conditions de réaction

Traitement

1. 1
   Températurewas refluxed for 11 h
2. 2
   AutreAfter being quenched with AcOH
3. 3
   Autrethe reaction mixture was partitioned between CHCl3/saturated aqueous NaHCO3 (60 mL×3/20 mL)
4. 4
   AutreThe product, obtained
5. 5
   Autreafter purification by silica gel column chromatography (hexane/EtOAc=1/1) of the organic layer
6. 6
   workup.DISSOLUTIONwas dissolved in saturated NH3 in MeOH (30 mL)
7. 7
   AutreEvaporation of the solvent
8. 8
   Autrefollowed by preparative TLC (hexane/EtOAc=1/1) purification
9. 9
   Autregave TKD-4-114 (49.6 mg, 74%) as a solid

Mode opératoire

A mixture of 13 (105 mg, 0.272 mmol) and DBN (101 μL, 0.815 mmol) in CH3CN (10 mL) was refluxed for 11 h. After being quenched with AcOH, the reaction mixture was partitioned between CHCl3/saturated aqueous NaHCO3 (60 mL×3/20 mL). The product, obtained after purification by silica gel column chromatography (hexane/EtOAc=1/1) of the organic layer, was dissolved in saturated NH3 in MeOH (30 mL), and kept at room temperature for 12 h. Evaporation of the solvent followed by preparative TLC (hexane/EtOAc=1/1) purification gave TKD-4-114 (49.6 mg, 74%) as a solid: mp 207-209° C.; UV (MeOH) λmax 264 nm (ε10800), λmin 235 nm (ε4800); 1H NMR (CDCl3) δ1.83 (3H, s, Me), 2.63 (1H, s, ethynyl), 3.47 (1H, br, OH), 3.88 (1H, d, Jgem=12.5 Hz, H-5′a), 3.96 (1H, d, Jgem=12.5 Hz, H-5′b), 5.91 (1H, dd, J1′,2′=1.1 Hz and J2′,3′=5.9 Hz, H-2′), 6.30 (1H, dd, J1′,3′=2.0 Hz and J2′,3′=5.9 Hz, H-3′), 7.16-7.17 (1H, m, H-1′), 7.44 (1H, d, J6,Me=1.1 Hz, H-6), 9.06 (1H, br, NH); FAB-MS m/z 249 (M++H). Anal. Calcd for C12H12N2O4.⅙H2O: C, 57.37; H, 4.95; N, 11.15. Found: C, 57.36; H, 4.69; N, 10.98.

Source