Réaction #217754

Équation de réaction

2,6-Diamino-9-(2-fluoro-β-D-arabinosyl)purine

adenosine

→

title compound

Rendement 100.0%

9-(2-Fluoro-β-D-arabinosyl)guanine

Rendement 100.0%

Solvants

Tris-HCl

Conditions de réaction

Traitement

1. 1
   AutreThe resultant reaction mixture
2. 2
   Filtrationwas subjected to filtration by use of a membrane
3. 3
   Filtrationfilter (PTFE, 0.5 μm)
4. 4
   Autrethe filtrate was prepared into 60 mL of an aqueous solution
5. 5
   AutreThe aqueous solution was purified by means of reverse-phase ODS column chromatography (80 mL, 0 to 2% aqueous acetonitrile)

Mode opératoire

2,6-Diamino-9-(2-fluoro-β-D-arabinosyl)purine (61.2 mg, 0.21 mmol) was dissolved in 50 mM Tris-HCl buffer solution (20 mL, pH 7.0), and adenosine deaminase (71 units) was added to the resultant solution, followed by stirring at room temperature for 1.5 hours. The resultant reaction mixture was subjected to filtration by use of a membrane filter (PTFE, 0.5 μm), and the filtrate was prepared into 60 mL of an aqueous solution. The aqueous solution was purified by means of reverse-phase ODS column chromatography (80 mL, 0 to 2% aqueous acetonitrile), to thereby yield 60.4 mg of the title compound as colorless crystals (yield: 100%).

Source